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Faculty for Biology, Chemistry, and Earth Sciences

Keylab Genomics & Bioinformatics

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(A. Weig, DNA-Analytik)

Microarrays

DNA-Microarrays are made of short (20 - 60 nt) DNA sequences attached to a solid surface, which can be used to measure the quantity of complementary DNA and RNA molecules in a sample. With modern microarray production technologies, hundreds of thousands of specific DNA probes can be synthesized on one single microarray. Major applications of DNA microarrays are:

  • Gene expression profiling: Analysis of the transcription of genes
  • Genotyping: Analysis of individual genomic DNA to allelic probes and structural variations;
  • Biodiversity and function: Analysis of communities and community functions
  • Biotechnology: Analysis of strain collections and knockout libraries 

Next-generation microarrays

State-of-the-art DNA in situ synthesis technologies allow the production of high-density microarrays using published or custom-designed sequences. Various microarray formats are available for different experimental designs, ranging from small microarrays (ca. 15,000 probes; 8 arrays per slide) to large microarrays (more than 1,000,000 probes). Up to 16 hybridization experiments can be performed in parallel, allowing cost-effective full transcriptome analysis not only in microorganisms but also in plants and animals.

Microarray probe design (selection of sequences) can be performed automatically of by using sequences provided by the user.

Microarrays developed in our lab:

  • Agaricus bisporus var. bisporus (H97), GPL20913;
  • Saccharomyces cerevisiae up/dnTAG array (including modified TAGs), GPL17535;
  • Aspergillus ruber (Eurotium rubrum), GPL16608;
  • EcoChip v3b, GPL13295;

All microarrays can be ordered by Agilent Technologies, please contact us to get the design ID.

Recommended literature

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